Cellecta’s Next-Generation Sequencing (NGS) Prep Kits for sgRNA, shRNA and DNA barcode libraries provide a protocol and reagents for the PCR amplification from genomic DNA and NGS sequencing of library-specific barcodes included in these libraries.
Each NGS kit includes sufficient reagents to process 12-48 DNA samples (depending on the amount of DNA/sample) with unique indexing primers to multiplex up to 12 samples per run in a single lane.
Supplementary indexing primersare also available, which increase multiplexing capacity to 24 samples per run in a single lane.
Cellecta NGS Prep Kits for analysis of sgRNA, shRNA and DNA barcode libraries are available for the following libraries listed below.
** When placing your order, ensure you receive theNGSPrepKit with the correct primers for your library by selectingboththetype of libraryandthevector for the library from thetwoseparatedrop-down menus.**
Catalog #
Description
Quantity Provided
LNGS-101
NGS Prep Kit for shRNA Libraries in pRSI12 (DECIPHER™)
Kit (48x 50ug preps)
LNGS-102
NGS Prep Kit for shRNA Libraries in pRSI16/17 (HGW)
Kit (48x 50ug preps)
LNGS-120
NGS Prep Kit for sgRNA Libraries in pRSG16/17 (KOHGW)
Kit (48x 50ug preps)
LNGS-130
NGS Prep Kit for sgRNA Libraries in pRSGT16/17
Kit (48x 50ug preps)
LNGS-200
NGS Prep Kit for Barcode Libraries in pRSI16/17 (CloneTracker™)
Kit (48x 50ug preps)
LNGS-300
NGS Prep Kit for Barcode Libraries in pScribe (CloneTracker XP™)
Kit (48x 50ug preps)
LNGS-310
NGS Prep Kit for Barcode Libraries in pM2.2/pM2.8 (CloneTracker XP™
Kit (48x 50ug preps)
LNGS-350
NGS Prep Kit for CRISPR-Barcode Libraries in pRSGScribe1cb (CloneTracker XP™ CRISPR)
Kit (48x 50ug preps)
LNGS-360
NGS Prep Kit for sgRNA Libraries in pRSGScribe (no clonal barcodes)
Kit (48x 50ug preps)
LNGS-400
NGS Prep Kit for Dual-sgRNA Libraries in pRSL10 (KADHGW/KIDHGW)
Kit (48x 50ug preps)
LNGS-900
NGS Prep Kit for sgRNA Libraries in LentiGuide (GeCKO, Brunello)
Kit (48x 50ug preps)
LNGS-905
NGS Prep Kit for sgRNA Libraries in LentiCRISPRv2 (GeCKO, Brunello Kinome)
